The BD Pharmingen™ Hoechst 33342 Solution is a reagent for the fluorescent staining of DNA nuclei in live fixed cells. On binding to DNA, the fluorescence greatly increases. Background: Hoechst 33342 is a specific fluorescent DNA stain that hoechst binds within the minor groove of double- stranded AT- rich regions. The technical data provided above is for guidance only. Aqueous solutions are stable for at. This product may be. , 1988; 949: 158 ; Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell hoechst sorter. 1 It datasheet emits blue fluorescence ( excitation 350 nm/ emission maximum datasheet 461 nm) when bound to double stranded DNA is useful as a marker of nuclei for cell hoechst cycle studies to distinguish nuclear morphology in. Hoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double- stranded DNA ( preferentially binds to A- T base pairs). Hoechst 33342 may be used to identify healthy datasheet apoptotic nuclear mor- phology for cell cycle studies. Description Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain. Hoechst 33342 is a cell- permeable fluorescent compound ( MW 561. datasheet on our website. This stain can be used on both live and fixed cells ( 1). Hoechst 33342 is a cell- permeable benzimidazole dye that stains DNA by binding to the minor groove of adenine hoechst thymine- rich sequences.
hoechst Storage: The lyophilized powder is stable for 1 year if kept below 0ºC with desiccant. 93) that is able to stain the DNA of eukaryotic and prokaryotic hoechst cells by datasheet binding with high affinity to the minor groove of. IC₅₀ & Target Dye reagent[ 1] DNA Stain[ 1] In Vitro Hoechst 33342 binds to adenine- thymine- rich regions of DNA in the minor groove. Hoechst- 33342 unlike other Hoechst dyes is more lipophillic due to an. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. Hoechst 33342 datasheet trihydrochloride is an A: T- specific DNA minor groove ligand that hoechst is a widely used fluorochrome datasheet for visualizing cellular DNA. Hoechst 33342 datasheet. Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double- stranded AT- rich hoechst regions. datasheet Hoechst 33342 emits blue ﬂ uorescence when bound to double stranded DNA. HoechstNone None None None EU OEL ( STEL) EU hoechst Skin Notation Chemical Name Austria Denmark Poland datasheet Switzerland HoechstNone HoechstNone None None None None None Handling Chemical Name Ireland Norway Spain OEL ( datasheet TWA) HoechstNone None None datasheet Avoid contact with skin eyes clothing. Biochim Biophys Acta hoechst 949, 158- 68. Search results for hoechst 33342 at Sigma- Aldrich. 93) that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor datasheet groove of AT- rich DNA sequences. Hoechst 33342 nucleic acid stain is a popular cell- permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. Excitation of the dye occurs datasheet around 350 nm with an emission maxima around 461 nm.
Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high- quality solution of Hoechst dye for fixed- live- cell fluorescent staining of DNA nuclei in cellular imaging techniques. Each vial of Hoechst 33342 contains 1 mL of aqueous solution at 200 μg/ mL ( hoechst catalog # 639). It is slightly more membrane per- meant than the Hoechst 33258 analog. Hoechst 33342 is a fluorescent nucleic acid stain useful for DNA labeling in fluorescence microscopy and flow cytometry. The compound hoechst is excited by ultraviolet light at around 350 nm emits blue/ cyan fluorescence light around an emission maximum at 461 nm.
Replicating or dividing chromosomes will not stain with Hoechst 33342 DNA dye.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. Tocris products are intended for laboratory research use only, unless stated otherwise. Hoechst 33342 Staining Dye Solution ( ab228551) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, microplate, cuvette, and flow cytometry applications.
hoechst 33342 datasheet
Assignment of DNA binding sites for 4', 6- diamidine- 2- phenylindole and bisbenzimide ( Hoechst 33258). A comparative footprinting study.